survival of normal and chlorine-stressed pathogenic and non-pathogenic vibrio parahaemolyticus under adverse conditions

conclusions chlorine-stressed cells of v. parahaemolyticus were more susceptible to adverse conditions than the non-stressed ones. pathogenic and non-pathogenic strains showed the same survival characteristics under the adverse conditions. these results should be considered in the development of food preservation measures. results findings of the current study revealed that the normal cells of pathogenic and non-pathogenic v. parahaemolyticus, as well as the chlorine-stressed cells of both strains behave similarly under adverse conditions. in addition, chlorine stress increased the susceptibility of pathogenic and non-pathogenic v. parahaemolyticus to incubation at 4°c, and the presence of high nacl content in the medium. however, chlorine stress did not significantly affect the thermal tolerance of pathogenic and non-pathogenic v. parahaemolyticus, and the susceptibility to incubation at -18°c. objectives the present study aimed to evaluate the survival of normal and chlorine-stressed cells of pathogenic and non-pathogenic v. parahaemolyticus under adverse conditions. materials and methods normal and chlorine-stressed cells of pathogenic and non-pathogenic v. parahaemolyticus were subjected to environmental stresses such as low storage temperature (4 and -18°c), high incubation temperature (50°c) and high nacl content (20%). viable counts were then made at various time intervals by surface plating on tsa-2.0% nacl, and the survival rates of the cells were determined and compared. background vibrio parahaemolyticus is an important human pathogen which can cause gastroenteritis when consumed in raw or partially-cooked seafood. the pathogenesis of v. parahaemolyticus is based on the presence of virulence factors: the thermostable direct hemolysin (tdh) and tdh-related hemolysin (trh), encoded by the tdh and trh genes, respectively.